Summary
Type: SPE
Application #: 302570
Catalog#: 730001 - SPE Cartridges, Chromabond, C18, 1ml, PP, 100mg, 100/pk
Detection: HPLC-MS
Conditions: Method A:
Sample application: 0,50 ml
Elution: Elution was performed with water containing increasing amounts of MeOH.
Evaporation: Flavonol glycosides eluted with 50% MeOH were evaporated to dryness. For analysis the residue was dissolved in 1 ml of a mixture of MeOH, acetone and water (50:25:25, v/v/v).
Method B:
Sample application: 0,50 ml of the solution was fractionated on the cartridge.
Elution: with water, 10%, 25% and 50% MeOH (2 ml each)
Evaporation: The last fraction containing the flavonol glycosides was evaporated and dissolved in 1,00 ml of a mixture of MeOH, acetone and water (50:25:25, v/v/v). A duplicate was prepared in the same way.
Authors: Sturm S Mulinacci N Vincieri F F Stuppner H
Source: Chromatographia 50 7/8 (1999) 438-433